Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/18/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Thomas Jefferson High School for Science and Technology |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Based on the visual displayed in the gel electrophoresis, there is a very faint band shown. The band being shown allows for reasonable clause of Wolbachia being present, but the faintness of the band which represents low DNA makes the confidence level low. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be postive for Wolbachia. |
Centipede – MJAR
Fruitfly – MJAR
Ant – MJAR
Mosquito – MJAR
Bumblebee – MJAR