Sample information
Picture	Entry by: Justin Y
Location
Collection date
Captive / Cultivated?	Wild-caught
Group	Thomas Jefferson High School for Science and Technology
Observations
Putative identification	Arthropoda Insecta Hymenoptera Vespidae Polistinae Polistes Polistes carolina
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Abdomen
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	MiniPCR
Buffer	TBE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	Medium
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1
Summary	The Polistes carolina was found to be negative for Wolbachia.