Sample information
Picture	Entry by: Pia fazaa
Location	No map location data available.
Collection date	09/10/2026
Captive / Cultivated?
Group	Penn State Lehigh Valley
Observations
Putative identification	Arthropoda Insecta
Methods
Extraction kit	Qiagen DNeasy Blood and Tissue Kit
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR Safe
Gel images
Protocol notes	DNA standard ladder 2.COI1+wol 3. control 4.+control 5. control 6.05+DNA 7 water control 8 empty
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Insecta was found to be negative for Wolbachia.