MSAH02

September 10, 2026

Sample information
Picture
Entry by: Ali Haynes
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Moves like a Catiplliar

started to shed white skin layer

was found hanging by a silk thread
Putative identification Arthropoda

Methods
Extraction kit Qiagen Blood and Tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

lane 1-latter

Lane 2- Shellback pixie

Lane 3- Mealy Bug

Lane 4- positive control

Lane 5- negative control

Lane 6- DNA control

lane7- water

 

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Based on the lab hypothesis that most insects have Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Arthropoda was found to be negative for Wolbachia.
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