Sample information
Picture	Entry by: Bella S
Location	No map location data available.
Collection date	09/10/2026
Captive / Cultivated?	Wild-caught
Group	Penn State Lehigh Valley
Observations	We caught it in the sweep net and it was running and jumping around.
Putative identification	Arthropoda Arachnida Araneae
Methods
Extraction kit	DNeasy (Qiagen) blood and tissue kit
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	1X TAE
DNA stain	SYBR-SAFE
Gel images
Protocol notes	Lane 1: DNA Standard Lane 2: MGDM#1 Wol + COI1 Lane 3: MGDM#2 Wol + COI1 Lane 4: Wol POS Wol + COI1 Lane 5: Wol NEG Wol + COI1 Lane 6: gDNA Control + COI1 Lane 7: Water Control Wol + COI1 Lane 8: EMPTY Lane 9: DNA Standard Lane 10: BSAL#1 Wol + COI1 Lane 11: BSAL#2 Wol + COI1 Lane 12: Wol POS Wol + COI1 Lane 13: Wol NEG Wol + COI1 Lane 14: gDNA Control + COI1 Lane 15: Water Control Wol + COI1 Lane 16: EMPTY
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary	The Araneae was found to be negative for Wolbachia.