Long-Tailed Giant Ichneumon wasp (Megarhyssa marcrurus)

September 10, 2026

Sample information
Picture
Entry by: Laurana E
Location No map location data available.
Collection date 11/05/2025
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

Original bug was lost – new bug distributed by professor
Putative identification Arthropoda Insecta Hymenoptera Apidae Apis Apis mellifera

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit.
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes
 DNA Standard
AYPHF1 + AYPHF7
AYPHF2 + AYPHF8
AYPHF3 + AYPHF9
AYPHF4 + AYPHF10
AYPHF5 + AYPHF11
AYPHF6 + AYPHF12
empty
DNA Standard
VALEE1 + VALEE7 – pictured insect
VALEE2 + VALEE8
VALEE3 + VALEE9
VALEE4 + VALEE10
VALEE5 + VALEE11
VALEE6 + VALEE12
empty

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No second band was present, therefore there was no Wolbachia contamination
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Apis mellifera was found to be negative for Wolbachia.
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