Genus Bombus

September 10, 2026

Sample information
Picture
Entry by: Sarah B.
Location No map location data available.
Collection date 09/10/2026
Captive / Cultivated? Wild-caught
Group Penn State Lehigh Valley
Observations

It was a frozen bumblebee, it was crunchy, its yellow and black.

 

 
Putative identification Arthropoda Insecta Hymenoptera Apidae Bombus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain SYBR Safe
Gel images
Protocol notes

 

 

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

it was high because the presence of the band in the gel suggests that it was Wolbachia positive.

 

 
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TTTNGGTGTGTGAGCAGGAATAATTGGAACTTCTTTAAGTTTATTAATTCGTACAGAATTAGGTAATCCTGGATCACTAA TTGGAGATGATCAAATTTATAACACTATTGTTACAGCTCATGCCTTTATTATAATTTTTTTTATAGTTATGCCAATTATA ATTGGAGGATTTGGTAATTGATTAATTCCTTTGATATTAGGAGCCCCTGATATAGCTTTCCCACGTATAAATAATATAAG ATTTTGATTACTACCCCCATCATTAACTTTATTAATTTCTAGAAGTATTGTTGAAAACGGAGCAGGAACAGGATGAACAG TTTACCCCCCTCTTTCCTCTAATATTGCCCATAGAGGATCTTCTGTTGATTTAGCTATTTTTTCTCTTCACCTTGCAGGA ATTTCCTCTATCCTAGGAGCAATTAATTTTATTACAACAATTATCAATATACGAATTAATAATATGTCATTTGATCAAAT ACCTTTATTTGTGTGAGCAGTAGGAATTACTGCTTTATTATTATTATTATCATTACCAGTTTTAGCTGGTGCAATTACTA TATTATTAACTGATCGGAATTTAAATACCTCTTTTTTTGACCCTGCTGGGGGAGGAGACCCAATTCTTTATCAACATTTA TTTTGATTTTNNNNNNNC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Bombus was found to be postive for Wolbachia.
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