JWO 06 – Northern Green Striped Grasshopper

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

Green/brown, large hind femurs, chewing mouth parts, black markings on inner side of legs.

Found on plant in HACC Pollinator Garden.
Putative identification Arthropoda Insecta Orthoptera Acrididae Chortophaga Chortophaga viridifasciata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 6 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Bright band in arthropod CO1 gene, no bands in Wolbachia specific gene.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chortophaga viridifasciata was found to be negative for Wolbachia.
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