JWO 15 – Goldenrod Soldier Beetle

September 11, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.29, -76.88
Collection date 08/31/2026
Captive / Cultivated? Captive / Cultivated
Group Commonwealth Charter Academy
Observations

On flower in HACC pollinator garden.

Black and yellow, striped on abdomen, wings have black spots on bottom half
Putative identification Arthropoda Insecta Coleoptera Cantharidae Chauliognathus Chauliognathus pensylvanicus

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 15 on gel image.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Vivid band for Arthropod CO1.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Chauliognathus pensylvanicus was found to be negative for Wolbachia.
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