JWO 19 – Common Green Bottle Fly

September 28, 2026

Sample information
Picture
Entry by: Jake O
Location
Approximate location: 40.32, -76.88
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Commonwealth Charter Academy
Observations

On a flower in HACC Pollinator Garden

Fly w/ shiny green & gold body.
Putative identification Arthropoda Insecta Diptera Calliphoridae Lucilia Lucilia sericata

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer 1X TBE
DNA stain GelGreen
Gel images
Protocol notes

Followed duplex PCR & Minione gel procedures. Refer to label 19 on gel image.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

Clear band for CO1, Wolbachia 162 rRNA gene has light band.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lucilia sericata was found to be postive for Wolbachia.
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