Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 42.00, 73.00 |
| Collection date | 10/03/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Berkshire Community College |
| Observations | It was under a rock, near tall grass, wild fruit, and a stream |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
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| Extraction kit | Monarch DNA extraction (NEB) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
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| Protocol notes | 1.) We used a DNA extraction protocol based on the insect adaption of New England Biolabs’ Monarch Spin gDNA Exraction kit (Product # T3010). 2.) The specimen was incubated for 40 minutes in a hot water bath at 56 degrees C. 3.) Details that differed from the written protocol (incubation time differences, accidental mistakes, etc.) were that extra 5ul of Proteinase K was added. |
Results |
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| Wolbachia presence | |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
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| Summary | |
(no title)
Yellowjacket Wasp
Pillbug
Umpachene Falls Caddisfly
Wolf Spider