Sample information

It was under a rock, near tall grass, wild fruit, and a stream

Methods

1.) We used a DNA extraction protocol based on the insect adaption of New England Biolabs’ Monarch Spin gDNA Exraction kit (Product # T3010).

2.) The specimen was incubated for 40 minutes in a hot water bath at 56 degrees C.

3.) Details that differed from the written protocol (incubation time differences, accidental mistakes, etc.) were that extra 5ul of Proteinase K was added.

4.) Our first PCR reaction was set up on Wednesday, October 21,2026 and:

• was a duplex reaction because we used both the Arthropod CO1 and Wolbachia 16s primers together
• used an annealing temperature of 49 degrees Celsius.
• used this Taq polymerase: New England Biolabs OneTaq Hot Start Quick-Load 2X Master Mix with Standard Buffer (#MO488S)

Results