DEM-TDB Hymenoptera

November 2, 2026

Sample information
Picture
Entry by: Tyra Brown
Location
Approximate location: 31.97, -81.16
Collection date 08/31/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Collection method/equipment:

Detailed description of the location of the collection site:

Detailed description of the habitat of the collection site:
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location In front of University Building
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I have a high level of confidence that my insect sample tested positive for Wolbachia because my gel electrophoresis results showed a band at approximately 438 bp for the Wolbachia 16S rRNA gene. My sample also showed the expected insect CO1 band at approximately 708 bp, which supports that insect DNA was successfully amplified. However, I would not say my confidence is 100% because I still need to consider the positive, negative, and no-template controls when interpreting the results. Overall, my results support my original prediction that my insect specimen would test positive for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be postive for Wolbachia.
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