Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 09/01/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The positive, negative, and no template controls were all successful and wolbachia appeared positive for Hymenoptera and negative for the 3 other accompanied insects during gel electrophoresis. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
BRI AJC Diptera
CON-ACS Lepidoptera
CON-EJC Orpthoptera
CONKAE-Orthoptera
CON-CGE Hymenoptera