DEM-NJR Hymenoptera

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 08/25/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

My partner and I used a sweep net to collect out specimens.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location head and lower body
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level is high because my predictions were proven correct during experimentation.
Wolbachia 16S sequence
AATAATCATTCGCTTAGAATTAG GAACTTGTAACTCATTAATTTTAAATGATCAAATTTATAATTCTATTGTTACTGGTCATGCTTTTATTATAATTTTTTTT ATAGTAATACCATTTATAATTGGAGGTTTTGGAAACTTTCTTGTACCCCTAATACTTGGTGCTCCTGATATAGCCTATCC TCGTATAAATAATATAAGATTTTGATTACTTCCCCCCTCTATTCTTCTATTAACAATCAGAAATTTTATTAATAATGGTG TAGGTACTGGTTGAACAGTCTACCCTCCCTTAGCTTCCAATATTTATCACAATGGTCCTTCCGTAGATTTAGCCATTTTT TCTCTTCATATTGCTGGAATATCCTCAATTCTTGGAGCAATTAATTTTATCTCTACTATCTTAAATATACATCATAAAAA TTTTTCTATAGATAAAATTCCTCTCTTAGTTTGAGCAATCTTAATTACTGCAATTCTTCTTTTATTATCACTACCTGTAC TTGCCGGAGCTATCACCATACTTCTAACTGATCGAAATTTANNACATCATTTTTTGATCCATCGGGAGGAGGGGACCCAA TTTTATACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
AATAATCATTCGCTTAGAATTAG GAACTTGTAACTCATTAATTTTAAATGATCAAATTTATAATTCTATTGTTACTGGTCATGCTTTTATTATAATTTTTTTT ATAGTAATACCATTTATAATTGGAGGTTTTGGAAACTTTCTTGTACCCCTAATACTTGGTGCTCCTGATATAGCCTATCC TCGTATAAATAATATAAGATTTTGATTACTTCCCCCCTCTATTCTTCTATTAACAATCAGAAATTTTATTAATAATGGTG TAGGTACTGGTTGAACAGTCTACCCTCCCTTAGCTTCCAATATTTATCACAATGGTCCTTCCGTAGATTTAGCCATTTTT TCTCTTCATATTGCTGGAATATCCTCAATTCTTGGAGCAATTAATTTTATCTCTACTATCTTAAATATACATCATAAAAA TTTTTCTATAGATAAAATTCCTCTCTTAGTTTGAGCAATCTTAATTACTGCAATTCTTCTTTTATTATCACTACCTGTAC TTGCCGGAGCTATCACCATACTTCTAACTGATCGAAATTTANNACATCATTTTTTGATCCATCGGGAGGAGGGGACCCAA TTTTATACCAACATTTATTTTGATTTTTTGG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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