DEM-JLK Diptera

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.98, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

In front of Lane Library, grassy area with tree bushes and shrubs.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

After the gel electrophoresis, there was only Insect CO1 gene and no Wolbachia gene.
Wolbachia 16S sequence
Arthropod COI sequence
TATATTAATTCGAACAGAATTAAGCCATCC AGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAGTTA TACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAATA AATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGTAC TGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCTTC ATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTACA TTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGCCGG GGCTATTACTATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCNGCANGAGGGGGAGACCCTATTTTAT ACCAANATTTATTTTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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