DEMKAP – Diptera

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The line of CO1 gene was thin and not as bright, a more above the other lines.
Wolbachia 16S sequence
Arthropod COI sequence
CTCATGNTTTTATCATANTTTTTTTTATAGTTAT ACCTATTATGANNGGAGGATTCGGAAATTGGCTTGTANCTNNNATATNANGCGCCCCTGATATANCNTTCCCTCGAATAA ACAATATAAGATTCTGGCNNNNCCCCCCTTCTCTAACCCTTCTTTTAATAANCAGGCTANTTGAAAATGGAGCCGGAACT GGTTGAACANNNNNNCCCCCNCTGNCAGCTAATATCGCACANAGAGGTGCTTCANTAGATTTANCTATTTTTANCCTTCN TCTAGCAGGAATCNGTTCNNTTCNCGGAGCAGTTAATTTTATTACTACTATAATCAATATACGTTCAATAGGCATAACAT TTGACCGANTANNNTTATTTGCCTGATCAGTTGNACTTACTGCTCTTTTACTTCTCCTATCCCTCCCAGNTNNANCANGA GCTATCNCAATGCTCCTCACTGATCGTAATATC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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