DEMDDB Blattodea

November 3, 2026

Sample information
Picture
Entry by: Dennis B
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Location: Behind the Science Center
  • Habitat: grassy low areas, low bushes, tall grass, trees
  • Method: sweep net and hands
  • Results: Dragonfly, kissing bug, cockroach, and spittlebug collected
Putative identification Arthropoda Insecta Blattodea Ectobiidae Blattella

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 1X TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My confidence level that the host insect is truly negative for Wolbachia is high because the negative finding is supported by three distinct layers of molecular evidence (PCR, Gel Electrophoresis, and DNA Sequencing), alongside successful positive controls.
Wolbachia 16S sequence
Arthropod COI sequence
AGAATATTAATCCGAGCAGAACTAAA TCAACCTGGTTCATTAATTGGAGATGATCAAATCTATAATGTTATTGTAACGGCTCACGCTTTTGTTATAATTTTCTTTA TAGTTATGCCAATTTTAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATATTAGGTGCTCCTGATATAGCATTTCCT CGTATAAATAATATAAGTTTTTGACTTTTACCTCCATCTCTATCTCTTCTATTAGCCAGTAGCCTTGTCGAAAGAGGAGC TGGGACTGGTTGAACCGTATACCCTCCCTTAGCTAGAGGAATTGCTCATGCTGGAGCTTCAGTAGATTTAGCTATTTTCT CATTACATCTTGCAGGTGTATCATCAATTTTAGGTGCCGTAAATTTTATTTCAACAATTATTAACATGAAACCAATTAAT ATAAGCCCTGAACGAATTCCTTTATTTGTTTGGTCAGTAGGTATTACTGCATTATTATTATTATTATCCTTACCAGTTCT TGCANGTGCAATTACAATATTATTAACAGACCGAAATTTAAATACTTCATTTTTTGACCCTGCNNNGGAGGTGATCCTAT TTTATATCAACATTTATTTTGATTTTTTGGTCACC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Blattella was found to be negative for Wolbachia.
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