GRE-AMG Diptera

November 3, 2026

Sample information
Picture
Entry by: Allison G
Location
Approximate location: 31.97, -81.16
Collection date 08/25/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Used sweep net to collect insect, found in shady area near trees and bushes.
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location savannah ga
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Highly confident in the results as it aligns with the class data results from previous students. After using the platform to research known data sequences for comparisons, it also aligns. It is not likely for the order Diptera to be infected with Wolbachia. 
Wolbachia 16S sequence
Arthropod COI sequence
TAGTTGGNACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCA TCCAGGATTTTTTATTAGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAG TTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGA ATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGG TACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTC TTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATT ACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGC CGGGGCTATTACTATACTATTAACAGATCGAAATTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
Report Inappropriate Post