DEMVMP-Hymenoptera

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • collected bugs from the Georgia Southern Community Garden
  • At time of collection, it was 1:41pm on Tuesday, September 1st, 2026
  • There were tall grasses, vines, bushes, small logs, and flowers
  • used a sweep net and our hands to collect bugs
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of an in-house reagents was used.

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence interval is medium because I predicted from the beginning of the lab that my insect would not have Wolbachia. Although, I was confident I am in the medium confidence level because this is my first time doing this procedure and I cannot with high confidence say I perfectly performed it.
Wolbachia 16S sequence
Arthropod COI sequence
AATAATCATCCGATTAGAACTAGGATCT TGTAATTCTCTAATTAATAATGATCAAATCTACAACTCCCTAGTTACTAGACACGCTTTTATTATAATTTTTTTTATAGT TATGCCCTTCATAATTGGGGGATTTGGAAATTTCTTAGTCCCCTTAATACTCGGCTCCCCTGATATGGCCTATCCTCGAA TAAATAATATAAGATTTTGACTTCTACCACCTTCTCTAACTCTTTTACTTATCAGAAGATTTATTAATAGAGGAGTGGGG ACAGGATGAACTATCTATCCACCACTAGCCTCCAATATTTTTCACAGAGGGGCCTCTATTGACCTATCTATTTTTTCCTT ACATATCGCCGGAATATCATCAATCTTAGGGGCTATTAATTTTATTTCTACAATCATTAACATACACCATAAAAATTTTA CTATAGATAAAATCCCCCTACTAGTCTGATCTATCCTTATTACAGCCATTCTTCTTTTACTTTCCCTNNCAGTTCTTGCA GGAGCTATTACAATATTACTAACTGACCGTAATTTAAACACCTCCTTCTTCGATCCCTCGGGAGGGGGAGATCCCATTCT ATATCAACATTTATTCTGATTTTTTGGTC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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