DEM-JDH

November 3, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

All equipment was handled by college students. it is quite possible that subjects cross-contaminated.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Gel band was not very bright and was slightly higher than expected.
Wolbachia 16S sequence
Arthropod COI sequence
GATTNTTCGNATANANNTAGGATCNT GNGATTCCTTNNTCTGTANTGACCAAGTTTATAATGTTTTANTTACTGGACATGCATTTATTATAATTTTTTTTATAGTT ATACCATTTATAATTGGAGGATTCGGAAACTTTCTTGTTCCCTTAATACTCGGCTCCCCTGATATANCTTACCCTCNAAT AAATAATATAAGATTTTGACTCTTGCCCCCCTCTCTCCTTCTTCTTCTATTAAGAAGATTCCTTGGGATAGGAGTAGGTA CAGGATGAACCATTTATCCCCCCTTAGCCTCAAATATTTTCCATANAGGCCCATCTATCGACCTTTCAATTTTTTCCCTC CACATTGCTGGTATATCTTCAATTTTAGGAGCAATTANTTTTATTGCTACAATCTTAAATATACATCATAAATCCTTTTC CCTTGATAAAATCTCTCTTTTAACTTGATCTATCTTAATTACTGCTATTCTGCTTCTTCTATCTTTGCCAGTTCTAGCTG GAGCTATTACAATACTTTTAACA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hymenoptera was found to be negative for Wolbachia.
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