DCW-MAT Lepidoptera

November 3, 2026

Sample information
Picture
Entry by: Destiny W.
Location
Approximate location: 31.97, -81.16
Collection date 09/01/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected at 10:50 am in a flower garden between Solms and Hawes Hall at Georgia Southern Armstrong campus. Collected using a sweep net.
Putative identification Arthropoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have high confidence in my results because the gel electrophoresis results showed clear DNA bands as expected, and the controls worked as expected.
Wolbachia 16S sequence
Arthropod COI sequence
CNAGAATATTAGGAACTTCTCNTTAGATTATTAATTCGAACTGAATTA GGAACTCCAGGTTCTTTAATTGGAGACGATCAAATTTATAATACTATTGTAACAGCTCATGCTTTTATTATAATTTTTTT TATAGTTATACCAATTATAATTGGAGGATTTGGAAATTGACTAGTACCGTTAATGCTTGGCGCCCCTGACATAGCTTTCC CCCGAATAAATAATATAAGATTTTGACTTTTACCCCCCTCTTTAACTCTTTTAATTTCAAGAATAATTGTAGAAAATGGG GCCGGAACTGGTTGAACTGTTTATCCACCTCTCTCATCTAATATTGCACATGGAAGAAGATCAGTTGATTTAGTAATTTT TTCCCTTCATTTAGCGGGAATTTCTTCTATTTTAGGAGCAATTAATTTTATTACTACAATTATTAATATACGAATTAATA ATATATCATTTGATCAAATACCATTATTCGTTTGAGCTGNAGGAATTACAGCTTTATTATTACTTTTATCATTACCAGTT CTAGCTGGAGCTATTACAATATTATTAACTGATCGAAACTTNNNACATCATTCTTTGATCCTGCTGGANNANGAGATCCN ATTCTATACCNACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lepidoptera was found to be negative for Wolbachia.
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