Sample information |
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| Picture |
|
|---|---|
| Location | Approximate location: 31.97, -81.16 |
| Collection date | 08/26/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Used a sweep net and plastic jar for collections. Specimen was found in grassy area near flowers, around 1:30 pm. |
| Putative identification | Arthropoda Insecta Hymenoptera Apidae Bombus |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | The control result were clear and expected, and my results match with no Wolbachia control. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
ATANTTGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACA TCCAGGAATATGAATTAATAATGATCAAATTTATAATTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAG TTATACCATTTATAATTGGAGGATTTGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTTCCNCGA ATAAATAATATTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTTTATTTACACCTAATGTAGG AACAGGTTGAACCGTATATCCCCCTTTATCATCTTATTTATTTCATTCATCACCNTCAGTAGATATTGCAATTTTTTCTT TANNTNNNNCAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATNGNNNCNATTATATTAATAAAAAATTTTTCATTA AATTATGATCAAATTACTTTATTTTCNTGATCTGTATGTATTACAGTATTATTATTAATTTTATCATTACCAGTTTTAGC NGGAGCAATTACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCAATANGNGGTGGAGATCCAANTT TATATCAACATTTATTTTGATTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Bombus was found to be negative for Wolbachia. |
CON-JRR Neuroptera
CON-RXB Hymenoptera
CONMED Diptera
CON-MAR Hemiptera
CON-CBS Blattodea