CONBJ Hymenoptera

November 4, 2026

Sample information
Picture
Entry by: Brie J.
Location
Approximate location: 31.97, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

oLocation and Habitat: Open grass field with lots of sun exposure, damp soil, and tall grass; located across from the Science Center

Collection Method: Sweep Net
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My controls were accurate. My specimen matched the Negative Control Template.
Wolbachia 16S sequence
Arthropod COI sequence
TAATTGGATCATCAATAAGATTATTAATTCGAATAGAACTTAGACA TCCAGGAATATGAATTAATAATGATCAAATTTATAANTCATTAGTTACTAGACATGCATTTTTAATAATTTTTTTTATAG TTATACCATTTATAATTGGAGGNTTNGGAAATTATTTAATTCCTTTAATATTAGGATCACCTGATATAGCTTTNCCNCGA ATAAATAATATTAGATTTTGATTATTACCTCCATCTATTTTTATATTATTATTAAGAACTNTATTTACACCNAATGTAGG AACAGGTTGAACNGTATATCCNCCNTTATCATCTTATTTATTTCATTCATCACCNTCAGTAGATATTGCAATTTTTTCTT TACATATAACAGGAATTTCTTCTATTATTGGTTCATTAAATTTTATTGNTACNATTATATTAATAAAAAATTTTTNNTTA AATTATGATCNNATTACTTTATTTTNNTGATCNGTATGTATTACAGNNTTATTATTAATTTTATCNNTACCAGTTTTAGC TGGAGCAATTACTATACTTCTTTTTGATCGAAATTTTAATACATCATTTTTTGATCCNNTANGNNGTGGAGATCCAATTT TATATCNACATTTATTTTGATTTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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