CONMJL – Orthoptera

November 4, 2026

Sample information
Picture
Entry by: MaryJane L
Location
Approximate location: 31.98, -81.16
Collection date 08/26/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found in a field with various tall grasses and a few scattered small bushes/trees.

Collection method: sweep net

 
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The results of my gel electrophoresis shows there was no DNA that was a match for Wolbachia found in my sect from the the Orthoptera order. I feel confident that my results are accurate, previous results of Orthopteras being infected with Wolbachia in the area I found mine was ~25% in a study sample from 2013-2015.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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