LSGBRI – DIPTERA

November 4, 2026

Sample information
Picture
Entry by: Lamar G
Location
Approximate location: 31.97, -81.16
Collection date 09/02/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer 0.25 TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level
  1. Diptera is the fourth lowest order in terms of prevalence of wolbachia infection
  2. My specimen is male
  3. No errors in experiments
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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