GGE-MAN Diptera

November 4, 2026

Sample information
Picture
Location
Approximate location: 31.97, -81.16
Collection date 09/02/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught outside the Georgia Southern University Armstrong campus, with an aerial net. The insect was found flying and was caught in the act of mating.
Putative identification Arthropoda Insecta

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Gel electrophoresis results showed that the insect tested negative for having the 16S Wolbachia gene.
Wolbachia 16S sequence
Arthropod COI sequence
AGGATAGTTGGAACTTCACTAAGTATATTAATTCGAACAGAATTAAGCCAT CCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTATAGT TATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTCGAA TAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCTGGT ACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTCTCT TCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAATTA CATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTAGCC GGGGCTATTACTATACTATTAACAGATCGAAATTTAAATACAAGTTTCTTTGACCCAGCAGGAGGGGGAGACCCTATTTT ATACCAACATTTATTTTGATTT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Insecta was found to be negative for Wolbachia.
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