Sample information |
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| Picture |
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|---|---|
| Location | Approximate location: 31.98, -81.16 |
| Collection date | 08/26/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | head, two wings, different textures of wings, leathery flexible forewings, simple front legs, mandibles mouth, missing pinchers, hind legs for jumping |
| Putative identification | Arthropoda Insecta Orthoptera |
Methods |
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| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | dna extraction kit of in-house reagents was used |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | no faint line on the gel electrophoresis |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
GAATTATTATTCGTGCCGAACTAGGA CAGCCGGGAACACTAATTGGAGATGATCAAATTTATAATACAATCATCACAGCACATGCATTTGTAATAATTTTCTTTAT AGTTATACCAATTATAATTGGTGGATTTGGAAATTGATTAATCCCCTTAATAATTGGGGCACCAGACATAGCATTTCCCC GAATAAATAACATAAGATTTTGACTTTTACCACCGTCATTGACTTTACTCATCTCGTCATCCATAATCGATAATGGAGCT GGTACAGGATGAACAGTCTACCCCCCACTAGCAAGAGTTATTGCTCACAGAGGTGCATCTGTAGACTTAGCCATCTTCTC TCTACATTTAGCGGGTGTTTCATCAATTCTAGGAGCAGTAAATTTTATTACAACAGTAATTAATATACGATCAGAAAGAA TAACATTAGATCAAACCCCCCTATTTGTATGGTCAGTCGCAATCACAGCATTATTACTTTTATTATCATTACCAGTGTTA GCAGGAGCCATCACAATATTATTAACAGATCGAAACTTAAATACCTCATTCTTTGATCCAGCAGGAGGGGGTGATCCAAT TTTATATCAACATCTATTTTGANTTTTTGGTC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Orthoptera was found to be negative for Wolbachia. |
CON-JRR Neuroptera
CON-RXB Hymenoptera
CONMED Diptera
CON-MAR Hemiptera
CON-CBS Blattodea