DEM-MARP Diptera

November 5, 2026

Sample information
Picture
Entry by: MarissaP
Location
Approximate location: 31.97, -81.16
Collection date 09/03/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Two sets of wings and hind wings
Putative identification Arthropoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

My confidence level is medium because I couldn’t tell if my results were to be expected or not.
Wolbachia 16S sequence
GTGGCGTGCTGATCCACGATTACTAGCGatcccttcttcgtgCACTCGAGTTGCAGAGTACAATCCGAACTGAgaTggcTTTTAAGGGATTAGCTTAGCCTCGCGACTTTGCAGCCCATTGTAGCCACCATTGTAGCACGTGTGTAGCCCACTCCATAAGGGCCATGATGACTTGACATCATCCCCACCTTCCTCCAGTTTATCACTGGCAGTTTCCTTAAAGTCCCCAGCATTACCTGATGGTAACTAAGGATGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAACACCTGTGTGAAACCCGGACGAACCGACCCTATCCCTTCGAATAGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
CAGGANAGTTGGNACTTCACTAAGTATATTAATTCGAACAGAATTAAGC CATCCAGGATTTTTTATTGGTGATGATCAAATTTATAATGTAATTGTTACAGCCCATGCTTTTATTATAATTTTTTTTAT AGTTATACCAATTATAATTGGTGGATTTGGAAACTGATTAGTTCCATTAATATTAGGAGCCCCTGATATAGCTTTTCCTC GAATAAATAATATAAGATTTTGACTTTTACCACCTTCTTTATCCCTTCTTCTAATAAGAAGCATAGTTGAAAACGGGGCT GGTACTGGATGAACAGTATATCCCCCTCTTTCTAGAAATATTGCCCATGCAGGAGCTTCTGTTGATTTAGCAATTTTTTC TCTTCATTTAGCTGGTATTTCTTCTATTTTAGGGGCTGTTAATTTTATTACTACAGTTATTAATATGCGATCTACTGGAA TTACATTTGATCGTATACCACTTTTTGTATGATCTGTAGTTATTACAGCCGTTTTACTTCTCCTATCCCTCCCAGTTTTA GCCGGGGCTATTACTATACTATTAACAGATCNAAATTTAAATACNAGTTTCTNNGACCCAGC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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