WIL-AAC HYMENOPTERA

November 5, 2026

Sample information
Picture
Entry by: AKEELIAH C
Location
Approximate location: 31.83, -81.63
Collection date 08/27/2026
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Insect has a body, adomen, thoras, 2 pairs of wings, hairy, 2 antennae, black and yellow in color, 6 legs.
Putative identification Arthropoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I have a high level of confidence in my results because they matched my original prediction that my insect would test negative for Wolbachia. The controls helped make the experiment more reliable by indicating whether the testing was working correctly. Since the results were what i expected, I feel confidence in the findings.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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