Sample information |
|
| Picture |
|
|---|---|
| Location | Approximate location: 40.56, -75.40 |
| Collection date | 09/10/2026 |
| Captive / Cultivated? | Wild-caught |
| Group | Penn State Lehigh Valley |
| Observations | Located on a flower when caught. Buzzing, yellow, and black colored. |
| Putative identification | |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | 1X TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | Lane 1- DNA Standard Lane 2- Arthropod #1 Lane 3- Arthropod #2 Lane 4- Arthropod #3 Lane 5- (+) control Lane 6- (-) control Lane 7- Wolbachia control Lane 8- Water control |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |


VCR-BRI
Spotted Cucumber Beetle – Diabrotica Undecimpunctata
WIL-AAC HYMENOPTERA
WIL-ACSM-Orthoptera.