Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/10/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | Acton-Boxborough Regional High School |
| Observations | Found in the woods |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera Formicidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | |
| Buffer | Other |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The fourth column from the top is the PCR of this ant. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | We also conducted a arthropod DNA test, where we received successful results. This indicates that our Wolbachia test was also likely successful. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Formicidae was found to be negative for Wolbachia. |
Water Boatman