Ant

January 28, 2022

Sample information
Picture
Location
Collection date 09/10/2021
Captive / Cultivated? Wild-caught
Group Acton-Boxborough Regional High School
Observations

Found in the woods
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system
Buffer Other
DNA stain GelGreen
Gel images
Protocol notes

The fourth column from the top is the PCR of this ant.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We also conducted a arthropod DNA test, where we received successful results. This indicates that our Wolbachia test was also likely successful.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be negative for Wolbachia.
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