K.3.1 (“Brown-banded cockroach”)

February 22, 2022

Sample information
Picture
Photos by: Sabrina G.
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer PBS
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

From the images of the gel alone, it’s difficult to tell whether the results indicate that the arthropod is Wolbachia positive or not.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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