Fruit fly #2

February 24, 2022

Sample information
Picture
Photos by: Amelia W.
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system
Buffer PBS
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

Controls worked.  Did not show Wolbachia nor Arthropod DNA bands in Gel Electrophoresis.
Wolbachia 16S sequence
438 bp
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
708 bp
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Drosophila melanogaster was found to be negative for Wolbachia.
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