A.5.2

March 6, 2022

Sample information
Picture
Photos by: Seojun P.
Location
Collection date 02/23/2022
Captive / Cultivated? Wild-caught
Group Singapore American School
Observations

Black ant.
Putative identification Arthropoda Hexapoda

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system
Buffer PBS
DNA stain
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be negative for Wolbachia.
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