Mosquito – Ricky

March 25, 2022

Sample information
Picture
Photos by: David R.
Location
Collection date 05/02/2021
Captive / Cultivated? Captive / Cultivated
Group South Plainfield High School
Observations

Male Mosquito – Culex Species
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain Other
Gel images
Protocol notes

P2 for ATL and N3 for AL

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The confidence level I have is 100% because the evidence of Wolbachia is in the data and gel.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hexapoda was found to be postive for Wolbachia.
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