Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 05/02/2021 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | South Plainfield High School |
| Observations | Male Mosquito – Culex Species |
| Putative identification | Arthropoda Hexapoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | P2 for ATL and N3 for AL |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The confidence level I have is 100% because the evidence of Wolbachia is in the data and gel. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman