Sample information
Picture
Location
Collection date	06/12/2022
Captive / Cultivated?	Wild-caught
Group	Richmond High School
Observations	In the gorge, under a log. In a wet area
Putative identification	Arthropoda Hexapoda Insecta Coleoptera Coccinellidae
Methods
Extraction kit	Thermoscientific GeneJet Genomic Purification
DNA extraction location	Abdomen
Single or Duplex PCR	Duplex Reaction
Gel electrophoresis system	Edvotek Gel Electrophoresis
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Unknown
Confidence level	Medium
Explanation of confidence level	Our insect DNA didn’t show.
Wolbachia 16S sequence
Arthropod COI sequence
Summary