Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 06/12/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Richmond High School |
| Observations | In the gorge, under a log. In a wet area |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera Coccinellidae |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Edvotek Gel Electrophoresis |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Medium |
| Explanation of confidence level | Our insect DNA didn’t show. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Vespidae Maculifrons
Tenebrio molitor
Wasp-Hymenoptera sp
Pollenia pediculata,
Phyciodes tharos