Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/14/2022 |
| Captive / Cultivated? | Captive / Cultivated |
| Group | The Summit Country Day School |
| Observations | From Carolina Biological, Stock 3/23 Drosophila were Miniature |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Drosophilidae Drosophila Drosophila melanogaster |
Methods |
|
| Extraction kit | Thermoscientific GeneJet Genomic Purification |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Our positive, negative, and water controls all worked and yielded the expected results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Drosophila melanogaster was found to be negative for Wolbachia. |
Water Boatman