Cluster Fly (ADB 1)

September 7, 2023

Sample information
Picture
Location
Collection date 05/02/2022
Captive / Cultivated? Captive / Cultivated
Group Woodford County High School
Observations

Malaise trap
Putative identification Arthropoda Hexapoda Insecta

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The purple band appeared below my DNA extraction.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Insecta was found to be postive for Wolbachia.
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