Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/19/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Diptera Bibionidae Bibio Bibio marci |
Methods |
|
| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level |
|
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Bibio marci was found to be negative for Wolbachia. |
Acheta Domesticus
Millipede
Cylindroiulus caeruleocinctus
Biting Midge
Eastern Red Centipede