Oniscus asellus EF1

May 17, 2022

Sample information
Picture
Photos by: Felicia E.
Location
Collection date 04/05/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

found in dead wood
Putative identification Arthropoda Malacostraca Isopoda Oniscidae Oniscus Oniscus asellus

Methods
Extraction kit Instagene Matrix
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

cytochrom oxidase dna band + wolbachia dna band
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TNANACANNACTCTNNNCGANANANTTGNGGGAGTCNCGCGCNNNCTCGTCCCTCTTNNNNNGATACCATCTNGAGATGTTGTGGTCTTTNTNTNACTGCCAGTGATAAACTGNATTANGGGGGAGGATGATNTNNTNCATGATTGTCCTTATTAANTGNGCTANCGNGTGCTACGATGAAAAGCTNCAATGGGCTGCAGAAGCGCGANGCTAAGAAAATCCCNTANTAGCCANCTCANGTCGGNNNAGACTCTGCGGGTCAAAAACANNANNTTTGANTNNCNANNNNNCNNGAANCANCATACCANGNTNAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Oniscus asellus was found to be postive for Wolbachia.
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