Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 04/05/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | found in dead wood |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Oniscidae Oniscus Oniscus asellus |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Fast Blast |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | cytochrom oxidase dna band + wolbachia dna band |
| Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT
BLAST at The Wolbachia Project BLAST at NCBI
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| Arthropod COI sequence | Download FASTA
Download AB1
TNANACANNACTCTNNNCGANANANTTGNGGGAGTCNCGCGCNNNCTCGTCCCTCTTNNNNNGATACCATCTNGAGATGTTGTGGTCTTTNTNTNACTGCCAGTGATAAACTGNATTANGGGGGAGGATGATNTNNTNCATGATTGTCCTTATTAANTGNGCTANCGNGTGCTACGATGAAAAGCTNCAATGGGCTGCAGAAGCGCGANGCTAAGAAAATCCCNTANTAGCCANCTCANGTCGGNNNAGACTCTGCGGGTCAAAAACANNANNTTTGANTNNCNANNNNNCNNGAANCANCATACCANGNTNAG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Oniscus asellus was found to be postive for Wolbachia. |
Water Boatman