Camponotus chromaiodes JA01

June 8, 2022

Sample information
Picture
Photos by: Jules A.
Location
Collection date 05/26/2022
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

near a dead tree, medium-sized, fast, found in the woods
Putative identification Arthropoda Hexapoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

lane 2 – Camponotus chromaiodes arthropod (COI)

lane 9 – Camponotus chromaiodes Wolbachia (16s)

The expected fragment for an arthropod is seen at 438 bp and the expected fragment for Wolbachia is seen at 708 bp.

Results
Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The is a band on the gel at 408 bp corresponding to the Wolbachia gene. However, the DNA went out for sequencing, but sequencing failed.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
TCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCGAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCC
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Hexapoda was found to be postive for Wolbachia.
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