Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/26/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | Pingry School |
| Observations | near a dead tree, medium-sized, fast, found in the woods |
| Putative identification | Arthropoda Hexapoda |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | lane 2 – Camponotus chromaiodes arthropod (COI) lane 9 – Camponotus chromaiodes Wolbachia (16s) The expected fragment for an arthropod is seen at 438 bp and the expected fragment for Wolbachia is seen at 708 bp. |
Results |
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| Wolbachia presence | Yes |
| Confidence level | Medium |
| Explanation of confidence level | The is a band on the gel at 408 bp corresponding to the Wolbachia gene. However, the DNA went out for sequencing, but sequencing failed. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
TCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCGAACTTTCAATACCATCGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTCCCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCCCCATCGATCTCCTTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTTCCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATTTTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATTACAGCTATTCTCCTTCTTCTGTCCCTACCTGTTCTAGCAGGAGCTATTACAATACTACTAACAGACCGAAATCTTAATACTTCATTTTTCGACCCTTCGGGAGGGGGAGATCC
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Hexapoda was found to be postive for Wolbachia. |
Water Boatman