Lycorma delicatula

June 9, 2022

Sample information
Picture
Photos by: Kelly C.
Location
Collection date 09/27/2021
Captive / Cultivated? Wild-caught
Group Pingry School
Observations
  • Red and brown wings with black spots.
  • Colored brown, black, white, and red.
  • Found in fall 2021 and frozen at -80ºC
Putative identification Arthropoda Insecta Hemiptera Fulgoridae Lycorma Lycorma delicatula

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The gel had a band at the Wolbachia site, and there was sequencing for the arthropod’s Wolbachia sample that was consistently above 40.
Wolbachia 16S sequence Download FASTA    Download AB1
GTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAGACTGCC AGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGG TGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTC GAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGC CCGTCAC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
TCCTCTATAAGAATAATCATTCGACTAGAGTTGGGATCTCCTGATTCACTAATTCTTAATGATCAAACTTTCAATACCAT CGTTACAAGTCATGCTTTTATTATAATTTTTTTTATAGTTATACCTTTTATAATTGGGGGATTTGGTAATTTTTTAATTC CCCTTATACTAGGATCTCCTGATATAGCTTACCCCCGTTTAAATAACATAAGATTTTGATTACTTCCTCCATCGATCTCC TTATTAATCCTAAGAAATTTTATTAATGAAGGATCTGGAACTGGTTGGACTGTCTACCCCCCTCTATCATCAAATACCTT CCATAGTGGCCCCTCTATTGACCTGACTATCTTTTCTCTCCATATTGCTGGTATATCCTCAATTATAGGAGCAATCAATT TTATTTCAACAATTATAAATATACATAATTCCAATATTTCCCTAGATAAAATTCCCTTATTAGTATGGTCTATTCTTATT ACAGCTATTCTCCTTCTTCTATCCCTACCTGTTCTAGCAGGAGCTATTACNATACTACTAACAGACCGAANTCTTAATAC TTCATTTTTCGACCCTTCGGGAGGGGGAGATCCTATTTTATACCA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Lycorma delicatula was found to be postive for Wolbachia.
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