Acanthosoma haemorrhoidale BK16

Sample information

Picture
Photos by: Benoit K.
Location
Collection date 04/30/2021
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera Acanthosomatidae Acanthosoma Acanthosoma haemorrhoidale

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Fast Blast
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level Medium
Explanation of confidence level

The line of BK16 shows no band of CO1, but the W-band alone is clearly visible.

Wolbachia 16S sequence Download FASTA    Download AB1
TTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTNCCATCAGGTAATGCTGRGKACTTTAAGGAAACTGCCAGTGATAARCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCYTTATGGAGTGGGCTACACACGTGCTACAATGGTGNCTACAATGGGYTGCAANGTSSNNANGCTNARSNNAWYCCYTAAAAGNCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTNCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCANGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGNTTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Acanthosoma haemorrhoidale was found to be postive for Wolbachia.
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