American House Spider

Sample information

Picture
Location
Collection date 08/30/2022
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

Mottled; speckled; biting; venomous

Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods

Extraction kit Wizard SV (Promega)
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Edvotek Gel Electrophoresis
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

The controls did not work, and the sample data did not show.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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