Formica Rufa AH1

October 26, 2022

Sample information
Picture
Photos by: Amélie H.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations
Putative identification

Methods
Extraction kit Instagene Matrix
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

We worked closely and you can clearly see the line of Wolbachia.
Wolbachia 16S sequence Download FASTA    Download AB1
GATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGG CTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAG TGCATGAAGTTGGAATCGCTAGTAATCGTG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary
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