Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/13/2022 |
| Captive / Cultivated? | Wild-caught |
| Group | KantiWil |
| Observations | Found on a wooden bench next to a meadow. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Rhyparochromidae |
Methods |
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| Extraction kit | Instagene Matrix |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Ability to see a clear and defined line at the expected spot. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
AACCAGGAATATTCATTGGAGATGATCAAATTTATAATGTAATTGTTACAGCACATGCATTTATTATAATTTTTTTCATAGTTATACCAATCATAATTGGAGGATTTGGAAACTGATTAATCCCACTAATAATTGGCGCCCCAGATATAGCCTTTCCACGAATAAATAATATAAGATTCTGATTATTACCACCATCATTAACCCTTTTAATAACTAGAAGATTAGTAGAAATAGGTGCAGGAACAGGATGAACTGTATATCCTCCTCTATCAAATAGATTATTCCATAGAGGAGCATCAGTAGACTTAGCAATCTTTTCATTACACTTAGCAGGAGTCTCTTCAATTTTGGGAGCCATTAATTTTATTTCAACTATTATAAATATACGCCCCTCTGGAATAACACCAGATCAAATCCCCCTATTTGTATGATCAGTAGGAATTACAGCCCTACTTTTGTTATTATCATTACCTGTATTAGCAGGAGCAATTACCATACTACTAACAGATCGAAATTTTAATACATCATTTTTTGATCCTACAGGAGGAGGGGACCCTATTCTCTACCAACATTTATTTTGATTTTTT
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Rhyparochromidae was found to be negative for Wolbachia. |
Water Boatman