Pisaura mirabilis LV2

October 26, 2022

Sample information
Picture
Photos by: Lea V.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

in a spider web between two branches
Putative identification Arthropoda Chelicerata Arachnida Araneae Pisauridae Pisaura Pisaura mirabilis

Methods
Extraction kit Instagene Matrix
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

The lines were not well visible on the gel. However, it was more visible than in the picture.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
AGTGGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTTATACCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATATTAGGAGCTCCTGATATATCATTCCCCCGAATAAATAATTTGTCTTTTTGACTTTTACCTCCTTCTTTATTTTTATTATTTATATCTTCTATAGTAGAAATAGGAGTTGGTGCTGGTTGAACAGTTTATCCTCCTTTAGCATCTACAGTTGGTCATATAGGAAGATCTATAGATTTTGCTATTTTTTCTTTACATTTAGCTGGGGCTTCTTCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Pisaura mirabilis was found to be negative for Wolbachia.
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