Sample information |
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Picture |
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Location | |
Collection date | 09/13/2022 |
Captive / Cultivated? | Wild-caught |
Group | St. Albans School |
Observations | I found this spider on my lab table in class. It was the middle of the day and 68 degrees F inside. |
Putative identification | Arthropoda Chelicerata Arachnida Araneae |
Methods |
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Extraction kit | DNeasy (Qiagen) blood and tissue kit |
DNA extraction location | Whole arthropod |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | MiniPCR |
Buffer | TBE |
DNA stain | GelGreen |
Gel images |
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Protocol notes | DNA Extraction: I made sure to crush the arthropod but it was too small to dissect. |
Results |
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Wolbachia presence | Yes |
Confidence level | High |
Explanation of confidence level | I had a very bright DNA band meaning that I got a lot of the correct DNA into my tube. I contaminated my negative controls so, even though those show as positive when they theoretically shouldn’t, I am more confident in my results because of that. |
Wolbachia 16S sequence | Download FASTA
Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGTTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCT
BLAST at The Wolbachia Project BLAST at NCBI
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Arthropod COI sequence | Download FASTA
Download AB1
CTGCTATAGTAGGAACTGCAATAAGAGTATTAATTCGTATAGAATTAGGACAATCAGGAAGATTTTTAGGTGATGATCATTTATATAATGTAGTTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCTATTTTGATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTACTTCCTTCTTCTTTGTTATTATTATTTATTTCTTCTATAGCTGAAATAGGAGTAGGGGCTGGATGAACAGTTTATCCTCCTTTATCTTCTAGTATTGGTCATATAGGGAGAGCTGTGGATTTTGCTATTTTTTCTTTACATTTAGCTGGTGCTTCATCTATTATAGGAGCTGTTAATTTTATTTCTACTGTAATAAATATACGGTGTGTTGGTATGACTTTTGAAAAAGTTCCATTATTTGTTTGGTCTGTTTTAATTACTGCTATTTTATTATTGTTATCTCTTCCTGTTTTAGCGGGAGCTATTACTATATTGTTGACGGATCGTAATTTTAATACTTCATTTTTTGATCCTGCAGGAGGGGGAGATCCTATTTTATTTCAACATTTATTTTG
BLAST at The Wolbachia Project BLAST at NCBI
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Summary | The Araneae was found to be postive for Wolbachia. |