Spider From Class

November 5, 2022

Sample information
Picture
Photos by: Alexander C.
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations

I found this spider on my lab table in class.  It was the middle of the day and 68 degrees F inside.
Putative identification Arthropoda Chelicerata Arachnida Araneae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

DNA Extraction: I made sure to crush the arthropod but it was too small to dissect.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I had a very bright DNA band meaning that I got a lot of the correct DNA into my tube. I contaminated my negative controls so, even though those show as positive when they theoretically shouldn’t, I am more confident in my results because of that.
Wolbachia 16S sequence Download FASTA    Download AB1
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGTTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CTGCTATAGTAGGAACTGCAATAAGAGTATTAATTCGTATAGAATTAGGACAATCAGGAAGATTTTTAGGTGATGATCATTTATATAATGTAGTTGTTACTGCTCATGCTTTTGTTATAATTTTTTTTATAGTAATACCTATTTTGATTGGAGGTTTTGGTAATTGATTAGTTCCTTTAATATTAGGGGCTCCTGATATAGCTTTTCCTCGTATAAATAATTTAAGTTTTTGATTACTTCCTTCTTCTTTGTTATTATTATTTATTTCTTCTATAGCTGAAATAGGAGTAGGGGCTGGATGAACAGTTTATCCTCCTTTATCTTCTAGTATTGGTCATATAGGGAGAGCTGTGGATTTTGCTATTTTTTCTTTACATTTAGCTGGTGCTTCATCTATTATAGGAGCTGTTAATTTTATTTCTACTGTAATAAATATACGGTGTGTTGGTATGACTTTTGAAAAAGTTCCATTATTTGTTTGGTCTGTTTTAATTACTGCTATTTTATTATTGTTATCTCTTCCTGTTTTAGCGGGAGCTATTACTATATTGTTGACGGATCGTAATTTTAATACTTCATTTTTTGATCCTGCAGGAGGGGGAGATCCTATTTTATTTCAACATTTATTTTG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Araneae was found to be postive for Wolbachia.
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