Halyomorpha Halys

November 6, 2022

Sample information
Picture
Photos by: Pierre A
Location
Collection date 09/13/2022
Captive / Cultivated? Wild-caught
Group St. Albans School
Observations
Putative identification Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Halyomorpha Halyomorpha halys

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls worked as expected and the results matched the gel electrophoresis.
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
CGTATCGAATTAGGACAGCCTGGAAGATTTATTGGTAATGATCAAATTTATAATGTAATTGTAACAGCACANGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTCGGTAATTGATTAGTCCCTTTAATAATTGGAGCACCTGATATAGCCTTCCCACGATTAAATAATATAANATTCTGATTATTACCCCCTTCATTAACTTTATTATTAATAAGAAGAATAGCAGAATCAGGAGCTGGGACAGGATGAACAGTCTATCCCCCCTTATCAAGTAATATCTCACATAGAGGAGCATCAGTTGATTTAGCTATTTTTTCCTTACATTTGGCTGGGGTTTCATCAATTTTAGGGGCAGTAAATTTTATTTCAACTATTATAAATATACGACCAACAGGTATAACCCCTGAACGAATCCCATTGTTTGTGTGATCAGTAGGAATTACTGCACTTCTTCTGTTATTATCCTTACCTGTGTTAGCAGGTGCTATTACTATACTATTAACNNACCGAAATTTTAATACCTCTTTTTTTGACCCATCAGGAGGAGGAGACCCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Halyomorpha halys was found to be negative for Wolbachia.
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