Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 05/21/2021 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Albans School |
| Observations | Bug in ethanol for over a year when analyzed Legs falling off / large |
| Putative identification | Arthropoda Hexapoda Insecta Orthoptera |
Methods |
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| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am highly confident in my insect not having Wolbachia because of the results from the gels. In the control for arthropod positive DNA, my test also showed positive. However while the control for Wolbachia positive was positive, and my test showed negative. Which showed that my insect was negative for Wolbachia DNA and why I am confident in that fact. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GNNTGANCTNNNNNNTAGGAACTTCCCTTAGATTATTAATTCGAGCTGAATTAGGNCAACCTGGATTTTTAATTGNANATGATCAAATTTATAATGTAATNNTAACTGCCCATGCNTTNGTTATAATTTTTTTNNNNNTAATACCAATTNNAATTGNNGGATTCGGAAATTGATTANNNCCNNNNATATTANGANNNCCNNANATANCATTCCCNCNAATAAATAANATNANATTTTNATTACTTCNNCCTTCATTANCACTNNNTTTANNANNAANNNNNNNNNANNATNNANCTGGAACTGNATGAACTGNTNANCNNNCTNNNNNNNNNNNGAATNNNNCNNGCNGNANGANNNNNANANTNNNNAATTTNNNNTTTACATTTNNNNGGNNNNNNNTNNATTTTANNNNNNNNAAATTTTATTACNNNNNNNNNNNANNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNANACNNNNATNNNNNNNANNNNNNNNTATTACNNNNNNNNNNNNNNNNNNNNCNNNACNNNNNNNNNNNNNNNNNNNNNNNATN
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Orthoptera was found to be negative for Wolbachia. |
Water Boatman