|Captive / Cultivated?
|St. Albans School
Bug in ethanol for over a year when analyzed
Legs falling off / large
|Arthropoda Hexapoda Insecta Orthoptera
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
|Explanation of confidence level
I am highly confident in my insect not having Wolbachia because of the results from the gels. In the control for arthropod positive DNA, my test also showed positive. However while the control for Wolbachia positive was positive, and my test showed negative. Which showed that my insect was negative for Wolbachia DNA and why I am confident in that fact.
|Wolbachia 16S sequence
|Arthropod COI sequence
| Download FASTA
GNNTGANCTNNNNNNTAGGAACTTCCCTTAGATTATTAATTCGAGCTGAATTAGGNCAACCTGGATTTTTAATTGNANATGATCAAATTTATAATGTAATNNTAACTGCCCATGCNTTNGTTATAATTTTTTTNNNNNTAATACCAATTNNAATTGNNGGATTCGGAAATTGATTANNNCCNNNNATATTANGANNNCCNNANATANCATTCCCNCNAATAAATAANATNANATTTTNATTACTTCNNCCTTCATTANCACTNNNTTTANNANNAANNNNNNNNNANNATNNANCTGGAACTGNATGAACTGNTNANCNNNCTNNNNNNNNNNNGAATNNNNCNNGCNGNANGANNNNNANANTNNNNAATTTNNNNTTTACATTTNNNNGGNNNNNNNTNNATTTTANNNNNNNNAAATTTTATTACNNNNNNNNNNNANNNNNNNNNNNNNGNNNNNNNNNNNNNNNNNANACNNNNATNNNNNNNANNNNNNNNTATTACNNNNNNNNNNNNNNNNNNNNCNNNACNNNNNNNNNNNNNNNNNNNNNNNATNBLAST at The Wolbachia Project BLAST at NCBI
|The Orthoptera was found to be negative for Wolbachia.